WTD: Prep for BAC sequencing
R. John Lye
rjl6n at virginia.edu
Wed Jan 17 10:21:17 EST 2001
"P. Rocha" wrote:
> I would be grateful if someone could make available a protocol for BAC DNA
> preparation for sequencing. For my purposes I would prefer mini/midi
> preps, but protocols for large preps are equally welcomed.
I recently used the protocol published at:
with good results. The only changes that I made were to grow up
the cells at 30 degrees rather than 37 degrees, and I only grew a
250 ml culture rather than their recommended 1 liter culture (and
scaled everything else appropriately).
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