WTD: Prep for BAC sequencing

R. John Lye rjl6n at virginia.edu
Wed Jan 17 10:21:17 EST 2001


"P. Rocha" wrote:

> I would be grateful if someone could make available a protocol for BAC DNA
> preparation for sequencing. For my purposes I would prefer mini/midi
> preps, but protocols for large preps are equally welcomed.

I recently used the protocol published at:

http://www.genome.ou.edu/DblAcetateProcV3.html

with good results.  The only changes that I made were to grow up
the cells at 30 degrees rather than 37 degrees, and I only grew a
250 ml culture rather than their recommended 1 liter culture (and
scaled everything else appropriately).

Good luck,
John






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