WTD: Prep for BAC sequencing

[R. John Lye]

"P. Rocha" wrote:

> I would be grateful if someone could make available a protocol for BAC DNA
> preparation for sequencing. For my purposes I would prefer mini/midi
> preps, but protocols for large preps are equally welcomed.

I recently used the protocol published at:

http://www.genome.ou.edu/DblAcetateProcV3.html

with good results.  The only changes that I made were to grow up
the cells at 30 degrees rather than 37 degrees, and I only grew a
250 ml culture rather than their recommended 1 liter culture (and
scaled everything else appropriately).

Good luck,
John