Cloning 5kb inserts

[Peter Ashby]

In article <3A655F8E.7598.3B007A at localhost>, vasant at PLANTPATH.KSU.EDU 
("Janakiraman Vasantharajan") wrote:

> Hi netters,
> I had attempted to clone a 5kb insert (from a genomic library) into 
> the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with 
> nosuccess. I am doing this only to sequence my inserts.  I do a blue 
> white screening, none of my white colonies contain the insert. One 
> of my colleagues suggested using the pUC 19 vector. 
> Any suggestions ?? 

You could try Asp718 instead of KpnI.

Peter

-- 
Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.