Cloning 5kb inserts
p.r.ashby at dundee.MAPS.ac.uk
Wed Jan 17 10:53:28 EST 2001
In article <3A655F8E.7598.3B007A at localhost>, vasant at PLANTPATH.KSU.EDU
("Janakiraman Vasantharajan") wrote:
> Hi netters,
> I had attempted to clone a 5kb insert (from a genomic library) into
> the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with
> nosuccess. I am doing this only to sequence my inserts. I do a blue
> white screening, none of my white colonies contain the insert. One
> of my colleagues suggested using the pUC 19 vector.
> Any suggestions ??
You could try Asp718 instead of KpnI.
Wellcome Trust Biocentre
University of Dundee
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