More detail about the method/prtocol being follwed will help in suggesting
At 02:56 PM 1/17/01 -0000, Janakiraman Vasantharajan wrote:
>I had attempted to clone a 5kb insert (from a genomic library) into
>the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with
>nosuccess. I am doing this only to sequence my inserts. I do a blue
>white screening, none of my white colonies contain the insert. One
>of my colleagues suggested using the pUC 19 vector.
>Any suggestions ??
>You can email me at
>vasant at plantpath.ksu.edu>Thanks in advance
>>>Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Dept. of Chemistry & Biochemistry
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003
Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu