Cloning 5kb inserts

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Jan 17 20:31:57 EST 2001


More detail about the method/prtocol being follwed will help in suggesting
remedies.


At 02:56 PM 1/17/01 -0000, Janakiraman Vasantharajan wrote:
>Hi netters,
>I had attempted to clone a 5kb insert (from a genomic library) into 
>the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with 
>nosuccess. I am doing this only to sequence my inserts.  I do a blue 
>white screening, none of my white colonies contain the insert. One 
>of my colleagues suggested using the pUC 19 vector. 
>Any suggestions ?? 
>You can email me at 
>vasant at plantpath.ksu.edu
>Thanks in advance  
>
>
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>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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