In article <3A655F8E.7598.3B007A at localhost>,
Janakiraman Vasantharajan <vasant at PLANTPATH.KSU.EDU> wrote:
>>I had attempted to clone a 5kb insert (from a genomic library) into
>the bluescript vector atleast 3 times (into Kpn I - Sma I sites) with
In my experience both KpnI and SmaI are fussy cutters--especially KpnI.
Are you sure it's cutting properly? Are you sure your insert has
appropriate ends? How have you prepared your inserts?
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England