human not mouse specific antibody for western
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Jan 18 10:06:29 EST 2001
Oliver.Politz at SCHERING.DE wrote:
:We are seeking information about a good antibody for detection of human derived
:proteins capable to distinguish between mouse and human therefor it should not
:react with mouse proteins.
:Any suggestions ?
Easy. Buy any anti-human goat IgG from Sigma, pass them
through the column with immobilized mouse IgG (can buy
ready to use from or can buy whole mouse IgG and couple
them to Sepharose using any of the existing easy methods.
Presto! Perfect human-specific reagent in large quantities
More information about the Methods