Help needed on separation of small oligonucleotides

Jim Lambert jim.lambert at uchsc.edu
Thu Jan 18 13:53:30 EST 2001


16% (19:1) Acryl:bis
7M urea
1X TBE

Mette og Michael wrote:

> Does anyone know how to obtain the best separation of small
> oligonucleotides on acrylamide. I need to separate oligonucleotides in
> the range of 20 to 40 mers. In the moment I am  running a 36 cm long 6%
> arylamide gel (1:19 acryl:bisacrylamide) with 8 M Urea in 1XTBE (ABI377,
> Applied Biosystems). Is there a more optimal acryl:bisacrylamide ratio
> for sep of small oligoes. Has anyone got any experience on that?
>
> Sincerely
> PhD student Mette Nyegaard
> KKA, Odense University Hospital
> Denmark
>
> ---






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