Protein degradation in E.coli

John Lum 97980722r at polyu.edu.hk
Thu Jan 18 21:35:58 EST 2001


Hi there,

    I'm expression a 120kDa protein as a GST-fused protein in E.coli
BL21. However, my western blot indicates multiple bands of smaller size
(25-70kDa), but the expected 120kDa protein is abscent. Can anyone give
me some advice on this matter?

Regards,
John






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