IP-Western

huangz at biowww.net huangz at biowww.net
Fri Jan 19 10:09:59 EST 2001


In an immunoprecipitation-western blot I used rabbit polyclonal antibody and protein A agarose beads(protein A is covalently bind to beads) to pull down antigen complex. In following western blot I used rabbit polyclonal antibody as primary antibody and HRP-anti rabbit IgG as secondary. The result turns out to be many bands, including IgG bands. Is there a good way to do IP-W and get clean band? Any suggestion are appreciated. 
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