5'cloning

pmoreno pmoreno at servidor.ibv.csic.es
Fri Jan 19 11:28:39 EST 2001


Hello¡
It´s easy if you know something of the gene; You can sintetize a primer of
3´ and sequence ( one reaction, only). You will know the 5´ extrem.

Pernille Winding wrote:

> Hi,
>
> Does anyone have a good suggestion on how to clone the 5' of a gene,
> when most of the 3' end is known (chick genome)? It would be nice to get
> the promoter region as well....






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