Hello¡ It´s easy if you know something of the gene; You can sintetize a primer of 3´ and sequence ( one reaction, only). You will know the 5´ extrem. Pernille Winding wrote: > Hi, > > Does anyone have a good suggestion on how to clone the 5' of a gene, > when most of the 3' end is known (chick genome)? It would be nice to get > the promoter region as well....