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Mouse cell Background in monoclonal staining

Jaime Castellanos jcastellanos at hemagogus.ins.gov.co
Fri Jan 19 12:01:11 EST 2001

Hi all,
I am trying to do an immunocytochemical staining with monoclonal antibodies
on mouse sensory neuron cultures, using ABC technique. But there is a strong
background in controls without primary antibody. Positive controls are
clean, but are there unspecific staining?
Secondary biotinilated anti-mouse may bind to mouse cells unspecifically?
How do block the background?
Thanks in avance,

Jaime Castellanos
Neurosciences Lab
Jaime E. Castellanos
Neurosciences Laboratory
National Institute of Health

Tel 57-1-222 05 77 Ext 433 - 502
Fax 57-1-222 30 55


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