Mouse cell Background in monoclonal staining

Jaime Castellanos jcastellanos at hemagogus.ins.gov.co
Fri Jan 19 12:01:11 EST 2001


Hi all,
I am trying to do an immunocytochemical staining with monoclonal antibodies
on mouse sensory neuron cultures, using ABC technique. But there is a strong
background in controls without primary antibody. Positive controls are
clean, but are there unspecific staining?
Secondary biotinilated anti-mouse may bind to mouse cells unspecifically?
How do block the background?
Thanks in avance,

Jaime Castellanos
Neurosciences Lab
NIH, Bogota COLOMBIA 
Jaime E. Castellanos
Researcher
Neurosciences Laboratory
National Institute of Health
Bogota, COLOMBIA

Tel 57-1-222 05 77 Ext 433 - 502
Fax 57-1-222 30 55


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