I am trying to do an immunocytochemical staining with monoclonal antibodies
on mouse sensory neuron cultures, using ABC technique. But there is a strong
background in controls without primary antibody. Positive controls are
clean, but are there unspecific staining?
Secondary biotinilated anti-mouse may bind to mouse cells unspecifically?
How do block the background?
Thanks in avance,
NIH, Bogota COLOMBIA
Jaime E. Castellanos
National Institute of Health
Tel 57-1-222 05 77 Ext 433 - 502
Fax 57-1-222 30 55