Is FORMAMIDE necessary for hybridizations?

R. John Lye rjl6n at virginia.edu
Mon Jan 22 08:57:34 EST 2001


Patrick Lynch wrote:

> Always wanted to ask this - is it best to carry out hybridizations in the
> presence or absence of 50% formamide?

I don't use formamide-based hybridization buffers. I prefer the
Church buffer (aqueous-based, phosphate buffered, high SDS).

John








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