chloramphenicol amplification

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Tue Jan 23 00:16:35 EST 2001


In article <3A6C4552.F01AA38F at slac.stanford.edu>,
  Mark Bowen <mbo at slac.stanford.edu> wrote:
> Could some one tell me about thepitfalls of using chloramphenicol to
> amplify plasmids in E. Coli.  I know the basic technique and the
> mechanism.  I'm looking for information on the effects on DNA quality
> and the possibility of increased mutation.
>

I don't believe that chloramphenicol amplification works for modern
high copy plasmids such as pUC, pGEM, Bluescript, etc.

Nick

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Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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