cDNA library in E.coli

Roger Becker rbecker at
Tue Jan 23 04:47:19 EST 2001

Dear collegues,

does anybody have experience with constructing a plasmid-based cDNA
library (not lambda phage-based). Especially I would like to know how to
proceed after the ligation and transformation step.
Please reply to my e-mail address. Thanks to all of you for paying
attention to my mail!


Dr. Roger Becker
Institute of Toxicology
Obere Zahlbacherstrasse 67
D-55131 Mainz
email: rbecker at
Phone: 0049-6131-393-4384
Fax: 0049-6131-393-3421

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