The latest revision of pDRAW32 uses all the published nearest neighbour
paramaters published by Dr. SantaLucia for calculation of Tm and Tm-PCR.
However, notice that the parameters have not been solved for all mismatched
dinucleotide basepairs yet.
pDRAW32 may be downloaded from
URL1: http://www.crosswinds.net/~acaclone/ or
"Reinhard" wrote in message
<000001c08457$9ecb03e0$06719495 at akm.akhwien.ac.at>...
Does anyone know an improved nearest-neighbor method for calculation of
melting temperatures, taking care of mismatched basepaires.
Dep. Clin. Microbiology, Vienna
reinhard.nadrchal at akh-wien.ac.at