Tm calculation

AcaClone acaclone at
Tue Jan 23 06:29:47 EST 2001


The latest revision of pDRAW32 uses all the published nearest neighbour
paramaters published by Dr. SantaLucia for calculation of Tm and Tm-PCR.
However, notice that the parameters have not been solved for all mismatched
dinucleotide basepairs yet.

pDRAW32 may be downloaded from
URL1: or

Best regards,
    "Reinhard" wrote in message
<000001c08457$9ecb03e0$06719495 at>...
    Does anyone know an improved nearest-neighbor method for calculation of
melting temperatures, taking care of mismatched basepaires.

    Reinhard Nadrchal
    Dep. Clin. Microbiology, Vienna

    reinhard.nadrchal at

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