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Destaining NBT-BCIP -northern-blot

francoise.martz at metla.fi francoise.martz at metla.fi
Tue Jan 23 07:39:43 EST 2001

Non-radioactive northern-blot, colorimetric NBT-BCIP detection:
To reprobe a northern membrane (nylon N+) using the DIG and the 
colorimetric system, I am removing the precipitate by incubation in DMF, 
50oC, but there are 2 problems:
- strong staining is not removed,
- I have the feeling that RNA are also removed (they are fixed by backing 
80oC, 2h).
So is there any other possible treatment to remove the color precipitate, 
that keeps the RNA on the membrane...?
Thanks for your help.

Françoise Martz
The Finnish Forest Research Institute
Rovaniemi Research Station
P.O. Box 16
96301 Rovaniemi, Finland
Tel: +358 16 336 4215 - Fax: +358 16 336 4640


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