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lipofectamine plus

Sophie Le Page slepage at umich.edu
Tue Jan 23 10:24:43 EST 2001

Hi all,

Has anybody out there tried staining cells with rhodamine-phalloidin (or
any other rhodamine tagged probe) after they have been transiently
transfected with lipofectamine plus? Recently I have tried this and have
got lots of background that I suspect may be the plus/DNA/lipofectamine
complex fluorescing.    I have done the staining many times with regular
lipofectamine and haven't had a problem with background.

Has anybody else had this happen??



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