Has anybody out there tried staining cells with rhodamine-phalloidin (or
any other rhodamine tagged probe) after they have been transiently
transfected with lipofectamine plus? Recently I have tried this and have
got lots of background that I suspect may be the plus/DNA/lipofectamine
complex fluorescing. I have done the staining many times with regular
lipofectamine and haven't had a problem with background.
Has anybody else had this happen??