Fw: destaining SDS-PAGE

Deanne Bell dbell at qnis.net
Tue Jan 23 17:48:28 EST 2001


Thanks Emir
This is good to know !
D. Bell

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> From: Emir <ekhatipo at NOSPAMmidway.uchicago.edu>
> To: methods at hgmp.mrc.ac.uk
> Subject: Re: destaining SDS-PAGE
> Date: Tuesday, January 23, 2001 2:41 PM
> 
> It depends on the destaining solution. Kimwipes work with the standard
> MeOH-based solution, whereas they don't work (i.e., don't bind the dye)
with
> 5H2O:4EtOH:1AcCOOH "fast-destain" solution some labs use.
> -Emir
> 
> 
> 
> "Dima Klenchin" <klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu> wrote in
> message news:94kvmr$jm8$1 at news.doit.wisc.edu...
> > Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> > :Dear All, my group uses Amberlite mixed resin to help destain
coomassie
> > :blue BUT only some types of the beads turn blue.  I wonder if anyone
> knows
> > :of a non-mixed product that I could use and save money.
> >
> > There is nothing wrong with kimwipes. Cellulose binds coomassie
> > quite tightly.
> >
> >         - Dima
> 
> 
> 


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