Protein assay problems
H.Veldman-2 at neuro.azu.nl
Wed Jan 24 04:38:57 EST 2001
> Does anyone have any experience with this kit or with similar methods?
> I'd also be interested in any other suggestions people might have about
> assaying my proteins - I have a max of ~5ul available for assaying from
> each sample.
> 'It might look like I'm doing nothing, but at the cellular level I'm
> really quite busy.'
The Bio-Rad DC Protein Assay (a variant of Lowry), when done in microtiter
plates, uses 5 ul of sample and will tolerate at least up to 1 % of SDS. It
is, however, incompatable with mercaptoethanol and you need a protein
concentration in the 0.1 to 1.5 mg/ml range.
Hope this might help,
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)
More information about the Methods