destaining SDS-PAGE

Sergio sergioal at bbm1.ucm.es
Wed Jan 24 11:55:16 EST 2001


Actually it works even using toilet paper.... so i guess it's the cellulose.
We also use the white foam from the roche enzyme containers. It works exactly
like the paper, but looks nicer.

Sergio

Michael Witty wrote:

> Dear Dima and Hiranya,
>                      is it cellulose or impurities in the tissue that
> absorb the coomassie?  Are kimwipes snowy white tissues or light brown low
> quality ones?  (we don't get them in the UK).  Sorry to keep labouring the
> point!  Mike.
>
> On Tue, 23 Jan 2001, Dima Klenchin wrote:
> >
> > There is nothing wrong with kimwipes. Cellulose binds coomassie
> > quite tightly.
> >         - Dima
>
> From: "Dr. Hiranya S. Roychowdhury" <hroychow at nmsu.edu>
>
> them thin and float them in the destain. Kimwipes or cellulosic materials,
> that do not fall apart in the destain, may also be used. This is nothing
> new and I wonder why more labs don't do this. The destain, if kept
> properly
> sealed, can be used several times. No change of destaining solution is
> needed and the gel is destained with a very clear background.






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