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K.Fushimi fushimi at ncnp.go.jp
Thu Jan 25 04:27:27 EST 2001

Hi all!

My friends have trouble in preparation of pCRblunt plasmid vector which
contains the ccdB
gene.  They store pCRblunt transformed E. coli with glycerol.
But when they use the stock, they often get wrong plasmid.
If you have some experience,  please give us advice!
Which E. coli strain dose cause better or worse results?
Can you store transformed E. coli with glycerol?

Kazuo Fushimi, Ph.D., PostDoc
National Institute of Neuroscience, NCNP, Japan(NIN)
Address: 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187, Japan
Telephone: 81-423-41-2711
E-mail: fushimi at ncnp.go.jp


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