pCRblunt

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Thu Jan 25 08:47:25 EST 2001



"K.Fushimi" wrote:

> Hi all!
>
> My friends have trouble in preparation of pCRblunt plasmid vector which
> contains the ccdB
> gene.  They store pCRblunt transformed E. coli with glycerol.
> But when they use the stock, they often get wrong plasmid.

Is the strain you use recA minus? Could your plasmid have undergone
rearrangements?
What is ccdB and could it be toxic to E. coli?

> Can you store transformed E. coli with glycerol?

I believe it is the ideal storage option (The max. amount is 40%, if I
remember correctly)

Susanne







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