Protein assay problems
thegrouchybeast at hotmail.com
Thu Jan 25 10:17:40 EST 2001
Henk Veldman wrote:
> The Bio-Rad DC Protein Assay (a variant of Lowry), when done in microtiter
> plates, uses 5 ul of sample and will tolerate at least up to 1 % of SDS. It
> is, however, incompatable with mercaptoethanol and you need a protein
> concentration in the 0.1 to 1.5 mg/ml range.
I tried that assay, but a 1:10 dilution of sample was right down at the
end of the calibration curve. Actually, I wasn't convinced it wasn't
just interference in the assay by SDS or Tris.
If I used neat protein in the assay, I wouldn't have enough to do any
replicates of the sample - I like to do triplicates at a minimum. Plus,
there would then be >1% SDS in the sample.
Thanks for the suggestion, though.
I see you standing there, far out along the way,
I want to touch you but, the night becomes the day.
I count the words that I am never going to say,
And I see you, in midnight blue. (ELO - Midnight Blue)
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