Is there a simple test for RNase and DNase free
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Fri Jan 26 12:14:47 EST 2001
You might check catalogs and/or contact vendors to ask what their QC
protocols are. For example I know the front of the NEB catelog contains
information on how they check for non specific nuclease contamination of
their restriction enzymes. I would think most vendors would be willing to
share how they assess QC of their products. As for checking for proteases,
I just came a across a reagent in Roche's catelog "universal protease
substrate" for doing QC to determine protease contamination.
Hope this helps.
>We would like to test a samples of the batch for
>"DNASE and RNASE free" to confirm they are
>molecular biology grade. Even the "protease free" test.
>Is there a simple test or protocol or kit I can use.?
Michael L. Sullivan, Ph.D
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax
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