Coupling of protein to resin

engelbert_buxbaum4 at my-deja.com engelbert_buxbaum4 at my-deja.com
Mon Jan 29 06:38:08 EST 2001


In article <001f01c089df$4be22cc0$995e6f83 at cam.ac.uk>,
  pl229 at medschl.cam.ac.uk ("Patrick Lynch") wrote:
> Hi all,
>
> Has anyone got suggestions for the best way to bind a protein to a
resin
> while maintaining its activity?

There is no way that is guaranteed to succeed, protein is not protein.
Several vendors (like Pharmacia or Pierce) flogg preactivated
gel-matrices with different chemistries, that you could try. Cyanogen
bromide activated sepharose is probably the most commonly used.

If you are dealing with a glycoprotein, you could try to link via the
carbohydrate moiety, this is most likely to leave enzymatic activity
intact.


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