Do you have anitsera recognizing the protein? For my protein, the antisera
does not inhibit enzyme activity and I've found I can bind active protein
quite nicely to a proteinG plate using antibodies. Of course, these sorts
of things depend a lot on the nature of your particualar protein.
>>Has anyone got suggestions for the best way to bind a protein to a resin
>while maintaining its activity?
>>Thanks in advance,
Michael L. Sullivan, Ph.D
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax