Coupling of protein to resin

[Michael L. Sullivan]

Do you have anitsera recognizing the protein?  For my protein, the antisera
does not inhibit enzyme activity and I've found I can bind active protein
quite nicely to a proteinG plate using antibodies.  Of course, these sorts
of things depend a lot on the nature of your particualar protein.

Mike


>Hi all,
>
>Has anyone got suggestions for the best way to bind a protein to a resin
>while maintaining its activity?
>
>Thanks in advance,
>
>Patrick
>
>
>---


Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax


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