NP-40 lysis

Warren Gallin wgallin at gpu.srv.ualberta.ca
Mon Jan 29 13:35:53 EST 2001


It should reflect the final concentration of the reagent, i.e. a 1:100
dilution of a 10% solution yields final 0.1% solution.  However, if this
is some in-house protocol that has never been published it is usually
worth it to double check with the original creator of the protocol;
strange things happen in writing down protocols.

Warren Gallin

Stephen Snowdy wrote:
> 
> When a protocol calls for say, 0.1% NP-40, does this mean 0.1% of the
> 10% solution (i.e. pretending the stock solution is 100%), or 0.1%
> taking into account that the stock is 10%?
> 
> TIA.
> --
> Stephen Snowdy






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