NP-40 lysis

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Mon Jan 29 16:40:37 EST 2001


In article <3A75D065.8F2D3A8F at med.unc.edu>,
  Stephen Snowdy <snowds at med.unc.edu> wrote:
> Ok, here's what Current Protocols (pg 16.20.11) says:
>
> For NP-40 Lysis Buffer:
> "1% (v/v) Nonidet P-40 (NP-40; from 10% stock)"
>
> I find this a little confusing since NP-40 is a trade name for a
> solution that is always 10%, but I'll certainly go with everyone's
> experience.  Thanks for the advice.
>

I used to be able to buy "neat" (i.e., 100%) Nonidet P-40. There is also
some confusion about the difference between Nonidet P-40 (commonly called
NP-40) and another detergent that is also called NP-40.

Nick

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Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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