plasmid kits opinions

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Mon Jan 29 19:34:13 EST 2001


In article <bykd6.14753$KP3.4639348 at news3.rdc1.on.home.com>, "EO" <eooe at hotmail.com> wrote:
>Hi,
>
>I asked this before but didn't get enough people to respond.
>It would be nice if all readers simply comment please.
>
>Here is the question again simlified:
>
>Please rate (your opinion) what kits are the 3 best
>for plasmid mini preps?
>
>1. ?
>2. ?
>3. ?

It really depends what you need the miniprep for.
In most cases, anything from simple alkaline pption,
with or without further cleanup, to a home made silica-based
minipreps, in batch or column format, will do just fine.

If price is not a consideration and you want your DNA
to be usable in just about any application, then Qiagen's
mini preps are quite good. A friend of mine is completely 
sold on Bio-Rad's diatomous earth-based kit (forgot the 
name - Prep A Gene?). In general, miniprep is not 
something that requires "quality" in a sense of DNA 
being chemically pure. Thus, "best" is what works 
best for YOU (and people are known to have different
preferences).

        - Dima






More information about the Methods mailing list