Hydrophobic Interaction Chromatography

WB Lai wbl20 at cam.ac.uk
Tue Jan 30 06:27:28 EST 2001

I am trying a difficult purification of a denatured protein (suspended in 8
M urea) on a HIC column.  Has anyone had any success with HIC purification
in the presence of urea?
My main problem is that the protein aggregates severely in chaotrope
concentration < 5M urea.
Any help is greatly appreciated.


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