NP-40 lysis

Dr. Duncan Clark Duncan at
Tue Jan 30 05:43:21 EST 2001

In article <3A75A624.8FDD7136 at>, the eminent Stephen Snowdy
at UNC Gene Therapy Center wrote
>When a protocol calls for say, 0.1% NP-40, does this mean 0.1% of the
>10% solution (i.e. pretending the stock solution is 100%), or 0.1%
>taking into account that the stock is 10%?

Protocols should really say 0.1% (v/v) which therefore would be 0.1ml
per 100ml of solution. Whenever they just say 0.1% it has to be 0.1%
(v/v) or that is what I assume :-)

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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