plasmid extraction kits poll

clarosa clarosa at biocomp.unl.edu
Tue Jan 30 09:55:14 EST 2001


I found that using cheapo diatomaceous earth protocol using chemicals from
scratch works just as well as any store bought .  A little less convenient but
cheaper.  Essential it is the same protocol as Gene Clean .   A google.com
search will reveal many varients of protocol.

Jim Gore wrote:

> We use the Qiagen Plasmid DNA Maxiprep Kit.  Sigma recently had a
> special price on their kit (at least in Canada) and we tried several
> test extractions with both companies' products for direct comparison.
> The Qiagen kit was much more consistent and gave a higher quality of
> DNA, at least in our hands.
>
> So much for my 2 cents worth!
>
> Jim
>
> On Thu, 25 Jan 2001 16:43:24 GMT, "EO" <eooe at hotmail.com> wrote:
>
> >Can all of you please help me - I am trying to do the following
> >survey online to get a better understanding of the following:
> >
> >Please list the top 3 companies that make the best plasmid extraction kits.
> >There are atleast 10 companies out there and if you have used kits or even
> >DNA extraction kits please respond.
> >
> >Quality and Results are very important and many labs have problems with
> >yeilds and manipulations of the plasmids afterwards.
> >
> >
> >






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