plasmid extraction kits poll

clarosa clarosa at biocomp.unl.edu
Tue Jan 30 09:57:55 EST 2001


Same as Prep a gene .. The key ingredient, cheap diatomaceous earth is Supelco
Celite 545-AW, cat no 20199-U

clarosa wrote:

> I found that using cheapo diatomaceous earth protocol using chemicals from
> scratch works just as well as any store bought .  A little less convenient but
> cheaper.  Essential it is the same protocol as Gene Clean .   A google.com
> search will reveal many varients of protocol.
>
> Jim Gore wrote:
>
> > We use the Qiagen Plasmid DNA Maxiprep Kit.  Sigma recently had a
> > special price on their kit (at least in Canada) and we tried several
> > test extractions with both companies' products for direct comparison.
> > The Qiagen kit was much more consistent and gave a higher quality of
> > DNA, at least in our hands.
> >
> > So much for my 2 cents worth!
> >
> > Jim
> >
> > On Thu, 25 Jan 2001 16:43:24 GMT, "EO" <eooe at hotmail.com> wrote:
> >
> > >Can all of you please help me - I am trying to do the following
> > >survey online to get a better understanding of the following:
> > >
> > >Please list the top 3 companies that make the best plasmid extraction kits.
> > >There are atleast 10 companies out there and if you have used kits or even
> > >DNA extraction kits please respond.
> > >
> > >Quality and Results are very important and many labs have problems with
> > >yeilds and manipulations of the plasmids afterwards.
> > >
> > >
> > >






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