Transgenic B-gal expression

Rudi van de Wetering ReplyToButNoSpamR.vandewetering at czzorlnm.azn.nl
Tue Jan 30 09:34:29 EST 2001


I'm analysing some mice expressing B-gal. I have a lot of blue-staining
which seems to be located at the membrane or extracellularly. However the
B-gal does not contain a membrane signal so it should remain cytoplasmic. Is
it a normal thing to see this membrane-like staining?

Rudi

I included two links to some pictures. They are not the best but i hope they
show what i mean.
http://baserv.uci.kun.nl/~rudiw/melkkl.jpg
http://baserv.uci.kun.nl/~rudiw/melk2.jpg








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