IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

In situ hybe emulsion

Peter Ashby p.r.ashby at dundee.MAPS.ac.uk
Tue Jan 30 10:20:54 EST 2001

In article <3A76D95F.3C2A4EFE at po-box.mcgill.ca>, Rachel 
<rgeddy at po-box.mcgill.ca> wrote:

> Dear Peter,
> Thanks for your reply. In this case, if my emulsion layer is too thick, 
> will
> the camera have difficulty seeing the silver grains on the section 
> through
> the emulsion?

Depends on the optics I suppose. If you can see the section then the 
emulsion is not too thick for viewing (it should be transparent after 
processing). I was concerned that by your description this could be the 
case. Apart from the emulsion situation are you seeing silver grains at 
all? Most people use darkfield illumination to see this but if it is 
dense it can be seen in brightfield too. You could try in 
sci.techniques.microscopy too, autoradiograpy is an old technique.


Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net