palmitoylation

Mark Bowen mbo at slac.stanford.edu
Tue Jan 30 04:18:56 EST 2001


Does anyone have experience with in vitro palimoylation?  I've tried the
reaction by adding palmitoyl CoA in DMSO to protein (25 kDa with 4 free
cys) in the presence of 1% octylglucoside.  This doesn't seems to be
working based on SDS-PAGE.

thanks

--
Mark Bowen







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