Taqman assay Question

Yu Yang Tang ytang at rmy.emory.edu
Tue Jan 30 16:25:40 EST 2001


 According to the ABI PRISM 7700 Sequence Detection System, I use
QIAampDNA Mini Kit purify the lymphocytes genomic DNA, but the DNA
concentration is only 50-60ng/ul ( total volume 200ul, 5 million cells).
Usually 1ug genomic DNA/well are required in taqman assay to detect HIV
provirus DNA. And the template volume is fixed (5ul/reaction). So I need
higher concentration template DNA. Who can help me solve this problem?


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