According to the ABI PRISM 7700 Sequence Detection System, I use
QIAampDNA Mini Kit purify the lymphocytes genomic DNA, but the DNA
concentration is only 50-60ng/ul ( total volume 200ul, 5 million cells).
Usually 1ug genomic DNA/well are required in taqman assay to detect HIV
provirus DNA. And the template volume is fixed (5ul/reaction). So I need
higher concentration template DNA. Who can help me solve this problem?