In article <7eN2OnDKOI+OfGeMjpIP84QZNn4a at 4ax.com>, the eminent H. V.
Taylor at Zentrum fuer Datenverarbeitung wrote
>I'd start with glycerol or ethylene glycol as they will more
>likely be compatible with >5M urea than other solvents.
Phenyl Sepharose CL4B with Urea gradient elution in a 20% ethylene
glycol buffer works for us OK.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....