Hydrophobic Interaction Chromatography
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Tue Jan 30 12:29:54 EST 2001
In article <7eN2OnDKOI+OfGeMjpIP84QZNn4a at 4ax.com>, the eminent H. V.
Taylor at Zentrum fuer Datenverarbeitung wrote
>I'd start with glycerol or ethylene glycol as they will more
>likely be compatible with >5M urea than other solvents.
Phenyl Sepharose CL4B with Urea gradient elution in a 20% ethylene
glycol buffer works for us OK.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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