Hydrophobic Interaction Chromatography

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Jan 30 12:29:54 EST 2001


In article <7eN2OnDKOI+OfGeMjpIP84QZNn4a at 4ax.com>, the eminent H. V.
Taylor at Zentrum fuer Datenverarbeitung wrote
>I'd start with glycerol or ethylene glycol as they will more
>likely be compatible with >5M urea than other solvents.

Phenyl Sepharose CL4B with Urea gradient elution in a 20% ethylene
glycol buffer works for us OK.

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






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