I create a lot of point mutants using site directed mutagenesis. The
plasmids are usually around 7kb and I use promega Pfu enzyme. I have been
subcloning the mutants after PCR to eliminate other mutations but have been
informed that people generally no longer bother as the enzymes are efficient.
If I no longer did the subcloning this would save me a lot of time and
money!! Any advice please as I am confused.
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