Any experience in improving blunt end liga

Nick Theodorakis nicholas_theodorakis at
Sun Jul 1 15:25:43 EST 2001

In <20010701143012.20030.qmail at>, fly wrote:
>Many thanks for your help.
>Would you please let me know:
>what does 5x buffer mean, does it contain half of what the 10x buffer has? I
>have only 10x buffer, how could i make 10x into 5x buffer?

If you were talking to me (it's hard to tell), I was referring to the buffer in
this documemt:

The 5X Buffer is:
250 mM Tris-Cl pH7.6
50 mM MgCl2
5 mM ATP
5 mM dithithreitol
25% (wt./vol) PEG8000

That bulletin gives some tips for blunt-end ligations.


Nick Theodorakis
nicholas_theodorakis at


More information about the Methods mailing list