ildus at ufacom.ru
Tue Jul 3 09:38:12 EST 2001
Does anybody know how to analyze very high expansion of CAG-repeats? We see
very many steps under the expanded alleles and several lines above them.
Sometime we cannot differentiate the dominant line, we cannot understand
what line the allele is. We use 8%, 29:1,3 PAAG (and tried to use 29:1 7-8%
PAAG) without denaturating conditions and BrEth with visualization in UV.
Amplification is specific.
Sincerely, Ildus K.
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