Restriction Enzyme Stability at 50 degC?

Wolfgang Schechinger wolfsc at
Wed Jul 4 02:37:48 EST 2001

Hi all, 

is there any info on stability / survival / star activity of restriction 
enzymes normally used at 37 degC when they are forced to 
work under more tropical conditions (i.e. in a double digest with 
SfiI at 50 degreesC?)

All input is welcome, 


PS I do not mean we have no thermostat here...

[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
e mail wolfsc at ibms dot sinica dot edu dot tw


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