Role of EDTA in Magnesium-containing buffers

Patrick Lynch pl229 at medschl.cam.ac.uk
Wed Jul 4 05:48:17 EST 2001


Hi all,

What's the point of having 1 mM EDTA in a buffer containing 10 mM magnesium?
Surely the role of EDTA is to chealate free Mg2+?  Does MgEDTA have some
inhibitory effect on enzyme activity as well?

Regards,

Patrick

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