Role of EDTA in Magnesium-containing buffers

Patrick Lynch pl229 at
Wed Jul 4 05:48:17 EST 2001

Hi all,

What's the point of having 1 mM EDTA in a buffer containing 10 mM magnesium?
Surely the role of EDTA is to chealate free Mg2+?  Does MgEDTA have some
inhibitory effect on enzyme activity as well?




More information about the Methods mailing list