wolfsc at ibms.sinica.edu.tw
Wed Jul 4 09:43:03 EST 2001
actually, dNTPs for a normal PCR mix should do the job, there's
nothing special about the kit, just all reagents in one box.
This issue was discussed here frequently and many people do
site directed mutagenesis analog to the quick change protocol
without buying the kit: Do a pfu pcr with the same primers,
DpnI digest, transform the mix, analyze 25 clones and that's it.
[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
128 Yen-Chio Yuan Rd. Sec.2
e mail wolfsc at ibms dot sinica dot edu dot tw
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