Oligo AS labeling with Klenow

Dr. Fabian Moebius Fabian.Moebius at uibk.ac.at
Thu Jul 5 02:25:08 EST 2001

I am considering to use KLENOW & RANDOM HEXAMERS to make Cy3 labeled
antisense DNA from my 50 bp oligonucleotides. I want to use these labeled
antisense oligos to check the sense oligos that I have spotted on a glass
DNA microarray.
Has anybody out there experience with labeling of 50 bp oligos with Klenow?
Will I get approximately even labeling?
Is there a protocol or reference?
Thank you very much for your help!

A. Univ.-Prof. Dr.med. Fabian F. Moebius
Facharzt für Pharmakologie und Toxikologie
Institut für Biochemische Pharmakologie
Peter-Mayr-Str. 1
A-6020 Innsbruck
Telefon ++43-512-5073155
Fax ++43-512-588627
Fabian.Moebius at uibk.ac.at


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