Non-enzymatic fragmentation of ss DNA?
pete.corish at ogt.co.uk
Thu Jul 5 03:51:35 EST 2001
Is anyone aware of a non-enzymatic procedure for the fragmentation of ss DNA analogous to the heavy-metal ion catalysis of RNA molecules? In particular, something that will reduce fragment length to below 100bp. Sonication is a possibility but I believe it doesn't typically produce such small fragments.
Failing that, what enzymatic procedures have been used successfully (DNaseI presumably is the best option)?
Many thanks for any assistance
Dr Pete Corish
Manager of Molecular Biology
Oxford Gene Technology (Operations) Ltd
Oxford BioBusiness Centre
Oxford OX4 4SS
Tel: +44 1865 405 130
Fax: +44 1865 405 120
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