bp to ug of DNA

Ashok Aiyar aiyar at ebv.mimnet.northwestern.edu
Thu Jul 5 08:42:02 EST 2001


On 4 Jul 2001 08:03:24 +0100,
    T V Naga Rama Chander (chander at kelvin.ncl.res.in) wrote:

> what is the conversion factor between bp of DNA and ug of DNA
> 
> i.e.
> 
> 1000bp DNA =    ? ug of DNA
> 
> any references/tips are welcome.

1 bp is approximately 650 daltons.                                              
It follows that one mole of a 1000 bp fragment is 650000 grams.                 
                                                                                
One mole has about 6.02*10e23 molecules (Avogardo's number)                     
                                                                                
Therefore one molecule of a 1000 bp piece of DNA weighs:                        
(6.5*10e5 / 6.02*10e23) = 1.08*10e-18 grams = 1.08 attograms                    
                                                                                
Later,                                                                          
Ashok     
-- 
Ashok Aiyar, Ph.D.
Assistant Professor                    email: a-aiyar at northwestern.edu
Department of Microbiology-Immunology           office: (312) 503-2524
303 E. Chicago Avenue, WARD 4-123                  lab: (312) 503-2542
Northwestern University, Chicago, IL 60611         fax: (312) 503-1339




More information about the Methods mailing list