bp to ug of DNA
aiyar at ebv.mimnet.northwestern.edu
Thu Jul 5 08:42:02 EST 2001
On 4 Jul 2001 08:03:24 +0100,
T V Naga Rama Chander (chander at kelvin.ncl.res.in) wrote:
> what is the conversion factor between bp of DNA and ug of DNA
> 1000bp DNA = ? ug of DNA
> any references/tips are welcome.
1 bp is approximately 650 daltons.
It follows that one mole of a 1000 bp fragment is 650000 grams.
One mole has about 6.02*10e23 molecules (Avogardo's number)
Therefore one molecule of a 1000 bp piece of DNA weighs:
(6.5*10e5 / 6.02*10e23) = 1.08*10e-18 grams = 1.08 attograms
Ashok Aiyar, Ph.D.
Assistant Professor email: a-aiyar at northwestern.edu
Department of Microbiology-Immunology office: (312) 503-2524
303 E. Chicago Avenue, WARD 4-123 lab: (312) 503-2542
Northwestern University, Chicago, IL 60611 fax: (312) 503-1339
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