I am afraid I got wrong Primer from company

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Jul 6 09:00:26 EST 2001


In <20010706011015.11262.qmail at ww02.jatek.com>, lee wrote:
>
>I run my PCR with primers from a well-known company.
>But when I cloned PCR product, then Sequense it for 4 times from both 
ends
>of it. I found the primer have one extra base, which will change my DNA's
>frame.
>Color electropherograms clearly showed an extra high peak there.
>I have contact with the company, they said they will check the primer by
>"mass spec". Is there any other reliable way to check it? Whick company
>could do it?

If you can find someone who still knows how to do Maxam-Gilbert 
sequencing, you can also sequence the primer directly.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu

 




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