Fw: Restriction Enzyme Stability at 50 degC?
dbell at qnis.net
Fri Jul 6 11:13:29 EST 2001
Schechinger at Academia Sinica wrote
: >is there any info on stability / survival / star activity of restriction
: >enzymes normally used at 37 degC when they are forced to
: >work under more tropical conditions (i.e. in a double digest with
: >SfiI at 50 degreesC?)
: My guess would be that start activity would not increase but half life
: of enzyme would decrease. I would reckon you will drop down to between
: 25and 50% of enzyme activity.
AFAIR star activity can occur if you get any evaporation during incubation
time (I think [glycerol] increases or something like that). At 50*C, I
imagine there will be alot of evap / condensation going on inside the tube.
I also think you just need to try it and see what happens
Molecular Markers Lab Technician
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