Restriction Enzyme Stability at 50 degC?

[Dr. Duncan Clark]

In article <3b499c75$1 at news.uia.ac.be>, the eminent Roland Hubner at
Lab. of Pathology, University of Antwerpen wrote
> that range seems to be what one would expect, but the activity of Spe I 
>is _ENHANCED_ 4-fold by incubation at 50?C (heat inactivated after 10 
>min at 65?C though)!

That just goes to show that there is still work to do on RE optimum
assay conditions. The same I think applies to Ban I, better at 50 than
37, despite the bug only growing at 37C.

Duncan
-- 
The problem with the gene pool is that there is no lifeguard.

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
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