Restriction Enzyme Stability at 50 degC?
Dr. Duncan Clark
news at hgmp.mrc.ac.uk
Mon Jul 9 08:00:56 EST 2001
In article <3b499c75$1 at news.uia.ac.be>, the eminent Roland Hubner at
Lab. of Pathology, University of Antwerpen wrote
> that range seems to be what one would expect, but the activity of Spe I
>is _ENHANCED_ 4-fold by incubation at 50?C (heat inactivated after 10
>min at 65?C though)!
That just goes to show that there is still work to do on RE optimum
assay conditions. The same I think applies to Ban I, better at 50 than
37, despite the bug only growing at 37C.
The problem with the gene pool is that there is no lifeguard.
More information about the Methods