migration of a non denaturant PAGE

Stefi~ xxxgifalone at tin.it
Tue Jul 10 13:38:04 EST 2001


I have two proteins with very similar mol weight (and very similar (but not
identical!) pI. When I do a SDS-PAGE and blotting I find bands at the same
position. As my antibodies are not very specific, I'd like to use blotting
on a non denaturant PAGE, hoping I'll have different migration. What do u
think?
Stefano
gifalone at tin.it

http://web.tiscalinet.it/gifalone
ICQ 110419911





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